Primer, dna detection method, and dna detection kit
AssigneeHitachi High-Tech Corporation
InventorJunko TANAKA
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Abstract
The present invention relates to a primer, a DNA detection method, and a DNA detection kit for use in nucleic acid amplification, and particularly in asymmetric nucleic acid amplification. More specifically, this invention relates to a primer for introducing a mutation into a test nucleic acid and amplifying the test nucleic acid, the primer contains, in its sequence, a mutation to be introduced into the test nucleic acid, so as to increase a proportion of single-strand forming bases in a probe binding region of the nucleic acid amplified from the test nucleic acid, at a temperature of the binding of the nucleic acid amplified from the test nucleic acid with the probe; and, a DNA detection method and a DNA detection kit with use of such primer.
Claims24
A method for detecting a target nucleic acid, the method comprising:
- amplifying a target nucleic acid-containing region of a test nucleic acid in the presence of a primer pair comprising a forward primer and a reverse primer, and a probe;
- and
- measuring binding of a nucleic acid amplified by the forward primer and the reverse primer, with the probe,
- wherein either the forward primer or the reverse primer, or both comprise, in the sequence, a mutation to be introduced into the target nucleic acid-containing region of the amplified nucleic acid, so as to increase a proportion of single-strand forming bases in a probe binding region of the amplified nucleic acid, at a temperature of the binding of the amplified nucleic acid with the probe, and
- by the amplifying step, the mutation is introduced into the target nucleic acid-containing region of the amplified nucleic acid.
A primer for introducing a mutation into a target nucleic acid-containing region of a test nucleic acid and amplifying the test nucleic acid, the primer comprising, in its sequence, a mutation to be introduced into the target nucleic acid-containing region of the test nucleic acid, so as to increase a proportion of single-strand forming bases in a probe binding region of the nucleic acid amplified from the test nucleic acid, at a temperature of the binding of the nucleic acid amplified from the test nucleic acid with the probe.
A kit for detecting a target nucleic acid, the kit comprising:
- a primer pair that comprises a forward primer and a reverse primer, for amplifying a target nucleic acid-containing region;
- and
- a probe that binds to a nucleic acid amplified with use of the forward primer and the reverse primer,
- wherein either the forward primer or the reverse primer, or both comprise, in the sequence, a mutation to be introduced into the target nucleic acid-containing region of the amplified nucleic acid, so as to increase a proportion of single-strand forming bases in a probe binding region of the amplified nucleic acid, at a temperature of the binding of the amplified nucleic acid with the probe.
Dependencies are inferred from the claim text.