Hybridisation detection
AssigneeEvonetix Ltd
InventorsMatthew James HAYES, Vijay NARAYAN, Raquel SANCHES-KUIPER
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Abstract
A method is provided for detecting a hybridisation indication indicative of an amount of hybridisation between a set of probe biomolecules provided at a reaction site and a set of sample biomolecules. The method includes supplying the set of sample biomolecules to the reaction site comprising the set of probe biomolecules. The reaction site is heated to cause dissociation of probe/sample-biomolecule-pairs previously hybridized when the set of sample biomolecules were provided to the reaction site. The hybridisation indication is generated based on detecting the dissociation of the probe/sample-biomolecule pairs caused by heating the reaction site.
Claims21
A method for detecting a hybridisation indication indicative of an amount of hybridisation between a set of probe biomolecules provided at a reaction site and a set of sample biomolecules, the method comprising:
- supplying the set of sample biomolecules to the reaction site comprising the set of probe biomolecules;
- heating the reaction site to cause dissociation of probe/sample-biomolecule-pairs previously hybridized when the set of sample biomolecules were provided to the reaction site;
- and
- generating the hybridisation indication based on detecting the dissociation of the probe/sample-biomolecule pairs caused by heating the reaction site.
Use of a thermal control chip, comprising a plurality of reaction sites and temperature control circuitry to independently control temperature for respective reaction sites, for increasing signal strength in detection of hybridisation between a set of sample biomolecules and respective sets of probe biomolecules provided at the respective reaction sites.
A method for detecting hybridisation between a set of sample biomolecules and respective sets of probe biomolecules provided at respective reaction sites of an apparatus comprising temperature control circuitry to independently control temperature for respective reaction sites;
- the method comprising:
- for each reaction site alternately selected as a selected reaction site, alternately performing a detection process comprising:
- controlling the temperature at the respective reaction sites to set the selected reaction site to a different temperature to other reaction sites;
- detecting a detection signal indicative of an amount of hybridisation between the set of probe biomolecules and the sample biomolecules, wherein the detection signal is incapable of resolving which particular site is the selected reaction site at which the hybridisation or dissociation occurred;
- and
- based on the detection signal, recording a hybridisation indication associated with the selected reaction site.
21-26. (canceled)
Dependencies are inferred from the claim text.