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AssigneesZencore Corporation, Shanghai ZhenGe Biotechnology Co., Ltd.
InventorsJinggong Chen, Wei Shao, Ying Xu, Yu Long, Jing Zhang, Lei Lei, Honghui Yuan, Chunxuan Mu, Kate Chen, Wei Wu, Weidong Hao, Jianxin Chen
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Disclosed herein are novel selectable markers and uses thereof. Specifically, provided herein are uses of nucleotide sequences encoding a glutamine synthetase (GS) derived from koala, ostrich, snake, pigeon, junglefowl, gerbil, or vesper bat, as selectable markers for identifying genomic loci with high transcriptional activity or host cells having high productivity of a protein of interest, and/or for accelerating the identification process. Related methods of screening, methods of production, and expression systems are also included.
Use of a nucleotide sequence encoding a glutamine synthetase (GS) as a selectable marker, wherein the GS is a koala GS, an ostrich GS, a snake GS, a pigeon GS, a junglefowl GS, a gerbil GS, or a vesper bat GS.
Use of a nucleotide sequence encoding a GS as a selectable marker, wherein the GS comprises a catalytic domain from a koala GS, an ostrich GS, a snake GS, a pigeon GS, a junglefowl GS, a gerbil GS, or a vesper bat GS.
A deoxyribonucleic acid (DNA) vector suitable for recombinant protein production or genomic integration, comprising a nucleotide sequence encoding a GS (aGS-encoding sequence), wherein the GS is a koala GS, an ostrich GS, a snake GS, a pigeon GS, a junglefowl GS, a gerbil GS, or a vesper bat GS.
A DNA vector suitable for recombinant protein production or genomic integration, comprising a nucleotide sequence encoding a GS (aGS-encoding sequence), wherein the GS comprises a catalytic domain from a koala GS, an ostrich GS, a snake GS, a pigeon GS, a junglefowl GS, a gerbil GS, or a vesper bat GS.
Dependencies are inferred from the claim text.