Carbohydrate composition and methods for producing alpha-glucooligosaccharides and use thereof
AssigneeBioneutra North America Inc.
InventorsYing HU, Vinti GOEL, Gonglai YAN, Jianhua ZHU
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Abstract
A method for preparing alpha-glucooligosaccharides (AGO) from starch employs simultaneous or sequential saccharification and multiple transfer reactions with two or more commercial glycosyltransferases, including Glyco Transferase, starch branching enzyme, cyclodextrin glycosyltransferases, and transglucosidase. Relative to common types of IMO, the chemical structure of AGO may be characterized by increased oligosaccharides with alpha-1,6 glycosidic linkages, or newly synthesized alpha-1,3 glycosidic linkages, or cyclic-alpha-1,4 glycosidic linkages with a medium dextrose equivalent (DE) between 20 and 30 to enhance the fiber content.
Claims22
A method for producing alpha-glucooligosaccharides (AGO), comprising the steps of:
- providing a source of starch;
- processing the source of starch to produce a liquefied starch solution with a mass concentration of between 10 to 45% (w/v) and a Dextrose Equivalent (DE) of between 7 to 15;
- adjusting a pH value of the liquefied starch solution to between 5.5-8.0;
- contacting the liquefied starch solution with one or more transfer reaction enzymes and incubating the liquefied starch solution at a temperature of between 55-65° C. for between 16-48 hours to produce a reaction solution;
- terminating the transfer reaction enzymes within the reaction solution;
- adjusting a pH value of the reaction solution to between 5.0-6.0;
- contacting the reaction solution with transglucosidase and incubating the reaction solution for between 24-56 hours;
- terminating the transglucosidase;
- and
- producing an AGO product.
Dependencies are inferred from the claim text.