US 2026/0226512 A1 · METHOD FOR IMPROVING RATE OF XYLOSE AND ARABINOSE UTILIZATION IN SACCHAROMYCES CEREVISIAE

Abstract

The provided is a method for improving the rate of glucose, xylose and arabinose utilization in Saccharomyces cerevisiae, comprising the following steps: using Saccharomyces cerevisiae with the ability to convert xylose and/or arabinose into ethanol, lactic acid, butanedioic acid, farnesene or isobutanol as a chassis cell to make the following mode of modifications in its genome: A. a mutation in the TRK1 gene for the potassium ion intracellular transporter protein, resulting in an increase in intracellular potassium ion/sodium ion ratio in Saccharomyces cerevisiae, and/or B. a mutation in the PUF2 gene for mRNA-binding protein or PUF2 gene knockout, resulting in PUF2 inactivation, deletion, reduced function, or down-regulated mRNA translation strength. The recombinant Saccharomyces cerevisiae constructed can utilize the cellulase hydrolysate comprising glucose, xylose and/or arabinose to produce ethanol, lactic acid, butanedioic acid, farnesene or isobutanol by fermentation, and has broad application prospects.

Claims20

Dependent claims

A method for improving the rate of xylose and arabinose utilization in Saccharomyces cerevisiae;

or a method for improving the rate of glucose and arabinose utilization in Saccharomyces cerevisiae;
or a method for improving the rate of glucose, xylose and arabinose utilization in Saccharomyces cerevisiae;
or a method for improving sodium salt tolerance of Saccharomyces cerevisiae;
or a method for improving fermentation of ethanol, lactic acid, butanedioic acid, farnesene or isobutanol;
wherein the method comprises the following steps:
fermenting said Saccharomyces cerevisiae with the ability to convert xylose and/or arabinose into ethanol, lactic acid, butanedioic acid, farnesene or isobutanol,
and optionally recovering the fermentation product;
wherein said Saccharomyces cerevisiae comprises one or both of the following modifications in its genome:
A. a mutation in an endogenous TRK1 gene for the potassium ion intracellular transporter protein, resulting in an increase in intracellular potassium/sodium ratio in Saccharomyces cerevisiae, and/or
B. a mutation in an endogenous PUF2 gene for mRNA-binding protein or PUF2 gene knockout, resulting in PUF2 inactivation, deletion, reduced function, or down-regulated mRNA translation strength.

7 more elements

A recombinant Saccharomyces cerevisiae cell, wherein the cell has the ability to convert xylose and/or arabinose into ethanol, lactic acid, butanedioic acid, farnesene or isobutanol;

and
wherein the cell has one or both of the following modifications in its genome:
A. a mutation in an endogenous TRK1 gene for the potassium ion intracellular transporter protein, resulting in an increase in intracellular potassium/sodium ratio in Saccharomyces cerevisiae, and/or
B. a mutation in an endogenous PUF2 gene for mRNA-binding protein or PUF2 gene knockout, resulting in PUF2 inactivation, deletion, reduced function, or down-regulated mRNA translation strength.

1 more element

Dependencies are inferred from the claim text.

Method for improving rate of xylose and arabinose utilization in saccharomyces cerevisiae

Life of this patent

Method for improving rate of xylose and arabinose utilization in saccharomyces cerevisiae

Life of this patent

Abstract

Claims20