Compositions and methods for purifying viral vectors
AssigneeNorth Carolina State University
InventorsStefano Menegatti, Wenning Chu, Shriarjun Shastry, Raphael Prodromou, Eduardo Barbieri
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Abstract
The present disclosure provides materials and methods related to the purification of viral vectors. In particular, the present disclosure provides compositions, and related methods, comprising peptide ligands capable of removing process-related impurities (e.g., host cell proteins, nucleic acids, and media components) and product-related impurities (e.g., product fragments, product aggregates, and inactive forms derived from product degradation by or association with other species in the cell culture harvest) from biological fluids during the production and purification of adeno-associated viruses (AAVs).
Claims37
A composition for purifying an adeno-associated virus (AAV) from a biological fluid comprising:
- (a) at least one peptide ligand that is at least six amino acids in length comprising the amino acid sequence X1-X2-X3-X4-X5-X6 (SEQ ID NO:
- 1), wherein X1 is F, S, K, or W;
- X2 is F, W, N, or K;
- X3 is N, F, E, A, I, or W;
- X4 is F, I, H, or K;
- X5 is F, I, N, or W;
- and X6 is K, F, I, S or A;
- and/or
- (b) at least one peptide ligand that is at least eight amino acids in length comprising the amino acid sequence X1-X2-X3-X4-X5-X6-X7-X8 (SEQ ID NO:
- 2), wherein X1 is F, I, or N;
- X2 is W, K, I, or A;
- X3 is N, A, W, I, or F;
- X4 is W, A, H, F, S or K;
- X5 is H, F, A, I, or K;
- X6 is H, F, A, or I;
- X7 is F, K, W, S, or I;
- and X8 is K, F, S, or I.
Dependencies are inferred from the claim text.