Pseudotyped viral particles for targeting tcr-expressing cells

A recombinant viral particle that is capable of binding to an antigen-specific T-cell receptor (TCR), the recombinant viral particle comprising on its surface a peptide (p) presented in the context of a major histocompatibility complex (MHC) molecule (pMHC complex).

A method of producing a viral particle that is capable of binding to a T-cell receptor (TCR), said method comprising culturing a packaging cell in conditions sufficient for the production of a plurality of viral particles, wherein the packaging cell comprises one or more plasmids comprising (i) one or more viral elements involved in the assembly of the viral particle, and (ii) a nucleotide sequence encoding a TCR-binding molecule.

A method of producing a viral particle that is capable of binding to an antigen-specific T-cell receptor (TCR), said method comprising culturing a packaging cell in conditions sufficient for the production of a plurality of viral particles, wherein the packaging cell comprises one or more plasmids comprising (i) one or more viral elements involved in the assembly of the viral particle, (ii) a nucleotide sequence encoding a peptide, and (iii) one or more nucleotide sequence(s) encoding a major histocompatibility complex (MHC) molecule, wherein the viral particles present the antigen-derived peptide in the context of the MHC.