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AssigneeSyngenta Crop Protection AG
InventorsRichard Paul DALE, Anthea Karin BATCHELOR, David BROCKLEHURST, Zane Lewis DUXBURY, Emma Catherine LINNEY
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The present invention relates to plants and pans thereof that have been modified to comprise a BioA and/or BIO3-BIO1 (BioDA) enzyme which confers at least partial resistance to compounds which inhibit the biotin synthesis pathway, such as herbicides, wherein in some cases the enzymes may be overexpressed to confer resistance, or may comprise modifications which confer resistance. The invention further relates to such modified BioA or BIO3-BIO1 enzymes, having modifications which improve resistance to such compounds, as well as polynucleotides and proteins encoding such enzymes. The invention also relates to methods of growing and propagating such plants, improving plant growth and controlling unwanted vegetation using such plants and parts thereof.
A method of controlling undesired vegetation in the vicinity of a plant or part thereof, or at the locus for growth of a plant or part thereof, wherein the plant or part thereof is modified to comprise a BIO3-BIO1 enzyme and/or BioA enzyme that provides the plant or part thereof with increased resistance to a compound which inhibits the biotin synthesis pathway relative to an unmodified reference plant, the method comprising:
A plant, or part thereof, modified to comprise a BIO3-BIO1 enzyme that provides the plant or part thereof with increased resistance to a compound which inhibits the biotin synthesis pathway relative to an unmodified reference plant, wherein the BIO3-BIO1 enzyme comprises an amino acid sequence having at least 30% identity to SEQ ID NO:1, and comprises an amino acid substitution at one or more of the following positions:
A plant, or part thereof, modified to comprise a BioA enzyme that provides the plant or part thereof with increased resistance to a compound which inhibits the biotin synthesis pathway relative to an unmodified reference plant, wherein the BioA enzyme comprises the following motif:
A modified BIO3-BIO1 enzyme having one or more mutations which provide increased resistance to a compound which inhibits the biotin synthesis pathway relative to an unmodified reference BIO3-BIO1 enzyme, wherein the modified BIO3-BIO1 enzyme comprises an amino acid sequence having at least 30% identity to SEQ ID NO:1, and comprises an amino acid substitution at one or more of the following positions:
Dependencies are inferred from the claim text.