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US 2026/0226483 A1Published application
New fucosyltransferases for production of 3fl
AssigneeDsm IP Assets B.V.
InventorManos PAPADAKIS
Life of this patent
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Abstract
The present disclosure relates to the biosynthetic production of the fucosylated Human Milk Oligosaccharides (HMO), 3-fucosyllactose, as well as to genetic engineering cells and α-1,3-fucosyltransferases suitable for use in said biosynthetic production, as well as to methods for producing 3FL.
Claims20
A genetically engineered cell capable of producing 3FL, comprising a recombinant nucleic acid sequence encoding a fucosyltransferase with α-1,3-fucosyltransferase activity, wherein the fucosyltransferase is selected from the group consisting of,
- a. Osc1, comprising the amino acid sequence of SEQ ID NO:
- 1, or a functional homologue thereof with an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 1,
- b. Bbac1 comprising the amino acid sequence of SEQ ID NO:
- 2, or a functional homologue thereof with an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 2,
- c. Murba1 comprising the amino acid sequence of SEQ ID NO:
- 3, or a functional homologue thereof with an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 3, and
- d. Bacbac1 comprising the amino acid sequence of SEQ ID NO:
- 4, or a functional homologue thereof with an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 4.
9 more elements
(canceled)
Dependencies are inferred from the claim text.