Methods for Inducing Modifications of Target Endogenous Nucleic Acid Sequences in Nucleuses of Eukaryotic Cells

Abstract

The present disclosure relates to methods for targeted genome editing in eukaryotic cells. In some embodiments, methods may comprise preparing an mRNA encoding a Cas9 protein and preparing a guide RNA, wherein the guide RNA comprises a crRNA and tracrRNA. Methods may further comprise introducing the mRNA and guide RNA into a eukaryotic cell, such as a non-human embryo, wherein the mRNA induces expression of the Cas9 protein in the non-human embryo, and wherein the Cas9 protein and guide RNA induce a modification at a target endogenous nucleic acid sequence. Methods may further comprise transferring the genome modified embryo into a foster mother and allowing the foster mother to produce a F0 animal having the modification at the target endogenous nucleic acid sequence.

Dependencies are inferred from the claim text.