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US 2026/0226464 A1Published application
Methods and compositions for modulating gene expression
AssigneeFlagship Pioneering Innovations V, Inc.
InventorsDavid Arthur Berry, Rahul Karnik, Laura Gabriela Lande
Life of this patent
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Abstract
The present disclosure provides compositions with a modulating gene expression and methods for modulating transcription
Claims21
-21. (canceled)
A method of decreasing expression of a gene within a cell, the gene being within an anchor sequence-mediated conjunction that comprises a first anchor sequence and a second anchor sequence, the method comprising a step of:
- contacting the cell with a composition comprising a targeting element or a nucleic acid encoding the targeting element, wherein the targeting element comprises a TAL array which targets the first anchor sequence, the second anchor sequence, or both of the first anchor sequence and the second anchor sequence,
- wherein the first and second anchor sequences each comprise a CTCF binding site.
A composition comprising a targeting element or a nucleic acid encoding the targeting element,
- wherein the targeting element comprises a Cas protein and gRNA which bind specifically to a first anchor sequence, a second anchor sequence, or both of the first anchor sequence and the second anchor sequence, wherein the first and second anchor sequences each comprise a CTCF binding site, wherein the CTCF binding site has the sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 2, and
- wherein the first anchor sequence, the second anchor sequence, and a gene are comprised in an anchor sequence-mediated conjunction.
1 more element
A composition comprising a targeting element or a nucleic acid encoding the targeting element,
- wherein the targeting element comprises a zinc finger array which targets the first anchor sequence, the second anchor sequence, or both of the first anchor sequence and the second anchor sequence, wherein the first and second anchor sequences each comprise a CTCF binding site, and wherein the CTCF binding site has the sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 2,
- wherein the first anchor sequence, the second anchor sequence, and a gene are comprised in an anchor sequence-mediated conjunction.
1 more element
Dependencies are inferred from the claim text.