Enhancement and stabilisation of proteolytic activity of proteases
AssigneePhoenix Eagle Company Pty. Ltd.
InventorGiuseppe Trigiante
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Abstract
The present invention relates to methods for the enhancement and stabilisation of the proteolytic activity of proteases, methods for producing compositions comprising proteases with proteolytic activity that has been enhanced and stabilised, compositions comprising proteases with proteolytic activity that has been enhanced and stabilised obtained or obtainable by the aforementioned methods, use of such compositions in the manufacture of medicaments and cosmetics, the use of such compositions in the treatment of diseases and disorders including wounds, and in cosmetic applications, and associated kits.
Claims6
A method for the enhancement and/or stabilization of the proteolytic activity of one or more cysteine proteases, wherein the method comprises:
- (i) contacting the one or more cysteine proteases with cysteine, wherein an active site cysteine residue of the one or more cysteine proteases is maintained in a reduced state;
- (ii) removing substantially all oxygen gas from the area surrounding the one or more cysteine proteases;
- and
- (iii) combining the one or more cysteine proteases and a carbomer such that the one or more cysteine proteases is non-covalently bound to the carbomer.
The method of claim 2, wherein the one or more cysteine proteases is packaged in a substantially oxygen-free atmosphere.
A method for producing a composition comprising one or more cysteine proteases with proteolytic activity that has been enhanced and/or stabilized, wherein the method comprises:
- (i) contacting the one or more cysteine proteases with cysteine, wherein an active site cysteine residue of the one or more cysteine proteases is maintained in a reduced state;
- (ii) removing substantially all oxygen gas from the area surrounding the one or more cysteine proteases;
- and
- (iii) combining the one or more cysteine proteases and a carbomer such that the one or more cysteine proteases is non-covalently bound to the carbomer;
- thereby producing a composition comprising one or more cysteine proteases with proteolytic activity that has been enhanced and/or stabilized.
Dependencies are inferred from the claim text.