Modified subtilisin proteins & uses thereof
AssigneeUniversity of Tennessee Research Foundation
InventorsJordan Cannon, Todd Reynolds
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Abstract
Modified subtilisin proteins and polypeptides are provided herein. Many of the modified subtilisin proteins and polypeptides exhibit an altered poly(L) lactic acid (PLLA) depolymerizing activity. Methods of depolymerizing PLLA methods of producing lactic acid, lactide and lactones from PLLA and methods of recycling plastic materials comprising PLLA with a modified subtilisin polypeptide are provided herein.
Claims34
A modified subtilisin polypeptide comprising at least one amino acid substitution at a position selected from S33, T33, S62, Q62, S98, R98, T99, Y99, D101, S101, Q103, Y104, P129, N130, T130, E156, S156, T159, S159, T162, A187, S189, F189, T215, G215, Y217, N218, and T218 of a subtilisin polypeptide comprising an amino acid sequence selected from the group of subtilisin amino acid sequences set forth in SEQ ID NO:1 and SEQ ID NO:2 and combinations thereof.
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Dependencies are inferred from the claim text.