Kappa-carrageenase cgk-gdsx478 for directionally preparing kappa-carrageenan disaccharide

Abstract

The present disclosure discloses a kappa-carrageenase Cgk-GDSX478 for directionally preparing a kappa-carrageenan disaccharide and relates to the technical field of biological enzymes. A nucleotide sequence of a gene Cgk-GDSX478 encoding kappa carrageenase is shown in SEQ ID NO: 1, and the amino acid sequence of the encoding kappa carrageenase Cgk-GDSX478 is shown in SEQ ID NO: 2. The present disclosure also provides a method for preparing the kappa-carrageenan disaccharide: adding kappa-carrageenase Cgk-GDSX478 into a kappa-carrageenan solution, and performing enzymolysis to obtain the kappa-carrageenan disaccharide, wherein enzymolysis reaction conditions are: a temperature is 4-70° C., a pH is 3.0-11.0, and a time is 30-1440 minutes. The kappa-carrageenan disaccharide obtained by performing degradation on kappa-carrageenan by the kappa-carrageenase of the present disclosure has high singleness, which is of great significance for industrial production of the kappa-carrageenan disaccharides.

Dependencies are inferred from the claim text.