Generation of induced pluripotent stem cells with polycistronic sox2, klf4, and optionally c-myc
AssigneeBeixcell (Beijing) Biotechnology Ltd.
InventorsPeng LIU, Sheng DING
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Abstract
Described herein a polycistronic expression cassettes and expression vectors that include a promoter operably linked to a nucleic acid segment that encodes a Sox2 and Klf4 polypeptide. The nucleic acid segment can also encode a c-Myc polypeptide. Expression of such polycistronic expression cassettes/vectors in host cells can reprogram the host cells to stem cells or other types of reprogrammed cells.
Claims15
A method to generate a host cell comprising a polycistronic expression cassette, which comprises a promoter operably linked to a nucleic acid segment, wherein the nucleic acid segment in the polycistronic expression cassette only encodes a Sox2 polypeptide in frame with a Klf4 polypeptide, as a single continuous open reading frame, the method comprising contacting a selected cell with the polycistronic expression cassette.
Dependencies are inferred from the claim text.