Protein complex and production process thereof

A protein complex, characterized in that its production process comprises:

• S1) culturing mesenchymal stem cells and creating a stressful environment using UV irradiation, conducting UV irradiation to stimulate the mesenchymal stem cells for 1 h to 30 h at an intensity of 10 μW/cm2 to 60 μW/cm2, with a UV wavelength of 290 nm to 340 nm;
• and
• S2) lysing the mesenchymal stem cells, followed by isolation and purification to obtain the protein complex;
• wherein the protein complex meets the following conditions:
• in SDS-PAGE detection, using 4% to 20% precast gel for sample separation and detection, sample bands are mainly distributed between 11 KD and 100 KD, wherein from high to low molecular weight, a first band is located between 75 KD and 100 KD, and a second band is located between 63 KD and 75 KD;
• 1) in size exclusion chromatography, under the conditions of exclusion chromatography with a flow rate of 0.2 mL/min and PBS as eluent, the elution volume for a first component peak is 12 mL to 13.2 mL, the elution volume for a second component peak is 15.2 mL to 17 mL, the elution volume for a third component peak is 17 mL to 20 mL, the elution volume for a fourth component peak is 29 mL to 31 mL, and the elution volume for a fifth component peak is 31 mL to 34 mL;
• and
• 2) in reversed-phase HPLC detection, under the conditions of loading volume of 60 to 80 μL, column temperature of 25° C. to 40° C., flow rate of 0.5 to 1 mL/min, detection wavelength of 220 to 280 nm, mobile phase A of TFA aqueous solution, mobile phase B of TFA in acetonitrile solution, and elution duration of 6 to 150 min, after separation, the peak appearance time is between 10-40 min, wherein characteristic peak 1 appears at 13-17 min, the components of the second sample group appear at 2-5 min, and characteristic peak 2 appears at 20-22 min;
• the protein complex comprises at least the following proteins:
• sp|P02768|ALBU_HUMANSerum albumin OS=Homo sapiens;
• and
• sp|P02787|TRFE_HUMANSerotransferrin OS=Homo sapiens;
• the mass of the above two proteins accounts for over 40% of the total protein complex mass.