Method for culturing muscle-derived cells, and culturing device containing muscle-derived cells
AssigneeIntegriCulture Inc.
InventorsMengxue LU, Miharu SHIDA, Ikko KAWASHIMA
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AssigneeIntegriCulture Inc.
InventorsMengxue LU, Miharu SHIDA, Ikko KAWASHIMA
Estimating the term…
The present invention aims to provide a novel method for culturing muscle-derived cells. In the method, muscle-derived cells and at least one type of cells selected from the group consisting of skin-derived cells, intestine-derived cells, heart-derived cells, brain-derived cells, stomach-derived cells, embryonic membrane-derived cells, liver-derived cells, kidney-derived cells, and lung-derived cells are co-cultured. Alternatively, muscle-derived cells may be cultured in the presence of a supernatant obtained from culturing at least one type of cells selected from the group consisting of skin-derived cells, intestine-derived cells, heart-derived cells, brain-derived cells, stomach-derived cells, embryonic membrane-derived cells, liver-derived cells, kidney-derived cells, and lung-derived cells.
A cell culture method comprising the step of co-culturing muscle-derived cells and at least one type of cells selected from the group consisting of skin-derived cells, intestine-derived cells, heart-derived cells, brain-derived cells, stomach-derived cells, embryonic membrane-derived cells, liver-derived cells, kidney-derived cells, and lung-derived cells.
A cell culture apparatus comprising a first culture container and a second culture container, the first culture container and the second culture container being connected so that a culture medium is circulated between the first culture container and the second culture container, the first culture container comprising muscle-derived cells, the second culture container comprising at least one type of cells selected from the group consisting of skin-derived cells, intestine-derived cells, heart-derived cells, brain-derived cells, stomach-derived cells, embryonic membrane-derived cells, liver-derived cells, kidney-derived cells, and lung-derived cells.
(canceled)
A cell culture method comprising the step of culturing muscle-derived cells in the presence of a culture supernatant obtained by culturing at least one type of cells selected from the group consisting of skin-derived cells, intestine-derived cells, heart-derived cells, brain-derived cells, stomach-derived cells, embryonic membrane-derived cells, liver-derived cells, kidney-derived cells, and lung-derived cells.
12-14. (canceled)
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