US 2026/0226404 A1 · METHOD FOR THE FERMENTATIVE PRODUCTION OF GUANIDINOACETIC ACID USING A MICROORGANISM COMPRISING A HETEROLOGOUS L-THREONINE 3-DEHYDROGENASE GENE (TDH) AND A GLYCINE C-ACETYLTRANSFERASE GENE (KBL)

Abstract

The present application is directed to a microorganism containing at least one heterologous gene coding for a L-arginine:glycine amidinotransferase (AGAT), at least one heterologous L-threonine 3-dehydrogenase gene (tdh), and at least one heterologous glycine C-acetyltransferase (2-amino-3-oxobutanoate coenzyme A ligase) gene (kbl). Also disclosed is a method for the fermentative production of guanidinoacetic acid (GAA) using such microorganism. The disclosure also relates to a method for the fermentative production of creatine.

Claims15

Dependent claims

A microorganism having an increased ability to produce L-arginine from L-ornithine compared with the ability of a wildtype microorganism or of a non-modified microorganism, the microorganism comprising:

at least one heterologous gene coding for a protein having the function of a L-arginine:glycine amidinotransferase,
at least one heterologous gene encoding a protein having L-threonine 3-dehydrogenase activity, and
at least one heterologous gene encoding a protein having a glycine C-acetyltransferase activity.

Dependencies are inferred from the claim text.

Facts

Application

19/151,363Filed 30 Jan 2024

Priority

EP 23154397.61 Feb 2023

Published

6 Aug 2026

Term ends

Not estimatedThe source records don’t support an estimate.

Classification30 codes

C12N 1/20
Microorganisms; Compositions thereof ; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of…:…
C12N 15/77
Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora: for Corynebacterium;…
C12N 9/0006
Oxidoreductases: acting on CH-OH groups as donors
C12N 9/1003
Transferases: transferring one-carbon groups

Method for the fermentative production of guanidinoacetic acid using a microorganism comprising a heterologous l-threonine 3-dehydrogenase gene (tdh) and a glycine c-acetyltransferase gene (kbl)

Life of this patent

Method for the fermentative production of guanidinoacetic acid using a microorganism comprising a heterologous l-threonine 3-dehydrogenase gene (tdh) and a glycine c-acetyltransferase gene (kbl)

Life of this patent

Abstract

Claims15