Methods for Producing and Cultivating High Productivity Algae Strains
OwnerDavid A. Hazlebeck, Agnieszka Pinowska +5inventors
InventorsDavid A. Hazlebeck, Agnieszka Pinowska, Jesse Traller, Alexander Beliaev, Eric Hill, Matthew Posewitz, Tyson Burch
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Abstract
The present disclosure describes a method for producing high oxygen tolerant algae strains. The methods include selectively growing algae cell cultures under high oxygen pressure conditions. Algae strains produced by these methods, as well as methods for cultivating them, are also disclosed.
Claims22
A method of photosynthetically or mixotrophically cultivating a high oxygen tolerant algae strain, the method comprising cultivating the strain in an aqueous medium comprising a pH of greater than 9.
A Nitzchia inconspicua strain GAI-337.
A method of producing a high oxygen tolerant algae strain, the method comprising:
- a) growing a cell culture of a parent algae strain in a medium comprising a pH of at least 9.0;
- b) gradually increasing oxygen concentration in the medium while diluting slow-growing cells out of the medium until cell growth is inhibited;
- c) decreasing the oxygen concentration in the medium;
- d) allowing the cell growth to recover;
- e) repeating steps a) through d) at least one time;
- wherein after step e), the cell culture comprises the oxygen tolerant algae strain.
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Dependencies are inferred from the claim text.