Algae biomass
AssigneeAlgenuity BV
InventorsAndrew Pearce Spicer, Alexander Findlay Pudney, Gino Schiano Schiano Di Visconte, Emma Ransom-Jones, Richard Thomas Smith
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Abstract
The invention relates to an algae biomass having particular values within a defined colour space. The invention further relates to a strain of Chlorella microalgae having a genetic mutation in genes encoding for specific subunits of magnesium chelatase, preferably magnesium chelatase subunit, and also relates to a method of producing a strain of Chlorella microalgae having such mutations.
Claims36
An algae biomass, wherein said biomass has a L* value in an L*a*b* colour space of greater than about 78.
The algae biomass of any preceding claim, wherein said biomass has a L* value in an L*a*b* colour space in the range of between 81.0 and 85.0;
- an a* value in the range of between 1.8 and 3.1;
- and a b* value in the range of between 16.0 and 19.0.
The algae biomass of any preceding claim, wherein said biomass has a protein content of at least 50% w/w.
The algae biomass of any preceding claim in the form of a powder or flour.
A Chlorella microalgae having a first mutation in at least one gene that encodes for phytoene desaturase or a subunit thereof, and wherein said Chlorella microalgae has a second mutation in at least one gene that encodes for magnesium chelatase or a subunit thereof.
The Chlorella microalgae of any preceding claim, cultivated in a heterotrophic growth mode.
The Chlorella microalgae of any preceding claim, cultivated:
- at a specific temperature;
- for a predefined period of time;
- without the presence of light;
- and
- in the presence of an organic carbon energy source.
A Chlorella microalgae comprising a genomic DNA sequence that is at least 50% identical to Sequence 2 (SEQ ID NO:
- 2).
A method of producing a Chlorella microalgae, the method comprising:
- a) obtaining a parent strain of Chlorella microalgae;
- b) performing mutagenesis of the parent strain of Chlorella microalgae;
- c) cultivating the mutated strain of Chlorella microalgae at a specific temperature, for a predefined period of time, and in the presence of an organic carbon source;
- and
- d) identifying and isolating mutants of the parent strain of Chlorella microalgae having a mutation in at least one gene that encodes for phytoene desaturase or a subunit thereof, and further having a mutation in at least one gene that encodes for magnesium chelatase or a subunit thereof.
A Chlorella microalgae strain selected from the following:
- (i) a Chlorella vulgaris strain designated CCAP 211/143 deposited under provisions of the Budapest Treaty at the Culture Collection of Algae and Protozoa (CCAP, SAMS Ltd., Scottish Marine Institute, OBAN, Argyll, PA37 1QA, United Kingdom) on Dec. 14, 2023 (Patent Deposit Designation of CCAP 211/143);
- (ii) a Chlorella sorokiniana strain designated CCAP 211/142 deposited under provisions of the Budapest Treaty at the Culture Collection of Algae and Protozoa (CCAP, SAMS Ltd., Scottish Marine Institute, OBAN, Argyll, PA37 1QA, United Kingdom) on Dec. 14, 2023 (Patent Deposit Designation of CCAP 211/142).
Dependencies are inferred from the claim text.