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US 2026/0226380 A1Published application
Killer yeasts to prevent spoilage of beer
AssigneeUniversity of Idaho
InventorPaul A. Rowley
Life of this patent
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Abstract
Variant K2 (K2v) killer toxins that inhibit the growth of diastatic yeast are provided. Also provided are engineered yeasts including K2v killer toxins, as well as methods of inhibiting diastatic yeast growth in fermented beverage products.
Claims22
A method of inhibiting diastatic yeast growth in a fermented beverage product, comprising:
- inoculating the fermented beverage product or a pre-fermented form thereof with an engineered yeast comprising a heterologous nucleic acid molecule encoding a variant K2 (K2v) toxin, wherein the K2v toxin comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 1, and comprises a methionine (M) at amino acid position 9, a glutamine (Q) at amino acid position 21, a lysine (K) at amino acid position 58, a glycine (G) at amino acid position 157, an isoleucine (I) at amino acid position 228, and a threonine (T) at amino acid position 259,
- wherein the amino acid positions are according to a reference K2v sequence set forth as SEQ ID NO:
- 1.
1 more element
An engineered yeast comprising a recombinant vector encoding a variant K2 (K2v) toxin, wherein:
- the K2v toxin comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 1, and comprises a methionine (M) at amino acid position 9, a glutamine (Q) at amino acid position 21, a lysine (K) at amino acid position 58, a glycine (G) at amino acid position 157, an isoleucine (I) at amino acid position 228, and a threonine (T) at amino acid position 259, wherein the amino acid positions are according to a reference K2v sequence set forth as SEQ ID NO:
- 1, and
- the recombinant vector comprises a nucleotide sequence at least 90% identical to SEQ ID NO:
- 3.
2 more elements
A Saccharomyces cerevisiae strain designated JM001, wherein a representative sample of cells of Saccharomyces cerevisiae strain JM001 has been deposited under ATCC Accession No. PTA-______.
Dependencies are inferred from the claim text.