Single domain serum albumin binding protein
AssigneeHarpoon Therapeutics, Inc.
InventorsRobert B. DUBRIDGE, Bryan D. LEMON, Richard J. AUSTIN, Luke EVNIN, Jeanmarie GUENOT
Life of this patent
Estimating the term…
Abstract
Disclosed herein are single domain serum albumins binding proteins with improved thermal stability, binding affinities, and robust aggregation profiles. Also described are multispecific binding proteins comprising a single domain serum albumin binding protein according to the instant disclosure. Pharmaceutical compositions comprising the binding proteins disclosed herein and methods of using such formulations are provided.
Claims20
A polynucleotide encoding a single domain serum albumin binding protein, the single domain serum albumin binding protein comprising complementarity determining region (CDR) 1, CDR2, and CDR3, wherein:
- (a) the amino acid sequence of CDR1 is as set forth in GFX1X2X3X4FGMS (SEQ ID NO:
- 1), wherein X1 is threonine, arginine, lysine, serine, or proline, X2 is phenylalanine or tyrosine, X3 is serine, arginine or lysine, and X4 is serine, lysine, arginine, or alanine;
- (b) the amino acid sequence of CDR2 is as set forth in SISGSGX5X6TLYAX7SX8K (SEQ ID NO:
- 2), wherein X5 is serine, arginine, threonine, or alanine, X6 is aspartic acid, histidine, valine, or threonine, X7 is aspartic acid, histidine, arginine, or serine, and X8 is valine or leucine;
- and
- (c) the amino acid sequence of CDR3 is as set forth in GGSLX9X10 (SEQ ID NO:
- 3), wherein X9 is serine, arginine, threonine, or lysine, and X10 is arginine, lysine, valine, proline, or asparagine,
- wherein X1, X2, X3, X4, X5, X6, X7, X8, X9, and X10 are not simultaneously threonine, phenylalanine, serine, serine, serine, aspartic acid, aspartic acid, valine, serine, and arginine, respectively.
Dependencies are inferred from the claim text.