Artificial polynucleotide molecules for enhanced stability and translation
OwnerYaniv ERLICH, Omer ZIV +2inventors
InventorsYaniv ERLICH, Omer ZIV, Andrew BEST, Christine MORDSTEIN
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Abstract
The present invention discloses artificial polynucleotide molecules comprising at least one open reading frame (ORF) and a synthetic 3′ terminus with increased stability and translational efficiency. The invention further discloses synthetic 3′ terminus compounds for linking to mRNA or mRNA-like molecules such as therapeutic mRNAs or replacing the conventional 3′ termini of said molecules. Further disclosed are methods for production and use as well as kits and pharmaceutical compositions comprising said artificial polynucleotide molecules and synthetic 3′ terminus compounds, preferably for use as therapeutic.
Claims69
An artificial polynucleotide molecule comprising or consisting of
- i. a polynucleotide M comprising or consisting of at least one open reading frame (ORF), and
- ii. a synthetic 3′ terminus comprising or consisting of at least one polynucleotide P, wherein, optionally,
- the 3′ end of the polynucleotide M (i) is linked to the synthetic 3′ terminus (ii), and/or
- said at least one polynucleotide P has a length of 4 nt and about 1000 nt, and comprises at least one DNA nucleotide, modified RNA, modified DNA or XNA nucleotide, non-natural backbone modification, modified nucleotide, modified backbone linkage, or any combination thereof.
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Dependencies are inferred from the claim text.