Extraction of nuclear and chromatin-bound proteins using volatile salts
AssigneeTalus Bioscience, Inc.
InventorsDaniele CANZANI, Andrea I. GUTIERREZ, Lindsay K. PINO, Alexander J. FEDERATION
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Abstract
The present disclosure relates to methods and compositions for peptide and protein extraction using solutions and buffers that contain volatile salts which are compatible with analysis by mass spectrometry.
Claims44
A method of extracting proteins from nuclei and chromatin, comprising contacting nuclei and/or chromatin with a solution comprising a volatile salt, separating insoluble chromatin from the solution, and collecting the solution supernatant, wherein the solution supernatant comprises proteins from nuclei and/or chromatin.
A method of extracting proteins from nuclei, comprising contacting nuclei bound to a solid support with a solution comprising a volatile salt, and then separating the nuclei bound to the solid support from the solution, wherein following the contacting, the solution comprises nuclear and/or chromatin-associated proteins.
Dependencies are inferred from the claim text.