Sulfonated-modified metal-organic framework material, preparation method therefor, and application thereof in yield increase of aryl glycoside compounds

Abstract

A sulfonated-modified metal-organic framework material, a preparation method therefor, and an application thereof in yield increase of aryl glycoside compounds are provided, and the sulfonated-modified metal-organic framework material is prepared through a reaction with 1,2-ethanedisulfonic acid for sulfonation modification where a zirconium-based MOF material Zr-BTB serves as a precursor. The sZr-BTB material prepared in the present disclosure is immobilized on a surface of Yarrowia lipolytica through physical adsorption to form a dense protective film, such that intracellular retention of relatively larger-molecule glycosidases prevents secretion but the efflux of small-molecule aryl glycoside compounds is not affected, thereby preventing the decomposition of a biosynthesized main product. Moreover, the present disclosure maintains stable adsorption of Yarrowia lipolytica under strongly acidic conditions, thereby facilitating yield increase of aryl glycoside compounds by fermentation, overcoming the obstacles of non-model microorganisms, and promoting the low-cost industrial biosynthetic production.

Dependencies are inferred from the claim text.