Sampling device

A method for performing sample analysis comprising:

• i) inserting a sample swab into a part of a single flow path in a cartridge wherein said single flow path is segmented into at least three compartments comprising:
• a first compartment comprising at least one substance for interacting with an item in a sample to be analyzed wherein said compartment is upstream of and in fluid communication with a reaction chamber but separated therefrom by a pierceable membrane or film;
• a second compartment comprising the reaction chamber adapted to accommodate the sample swab wherein said compartment comprises at least one movable shutter to control the retention of substance and/or sample in said reaction chamber;
• and
• a third compartment which is downstream of the reaction chamber, and through which said substance flows after having had contact with said sample, and which comprises a sensor strip on which said substance is analysed by a reader;
• ii) releasing said substance from said first compartment by piercing said membrane or film so that said substance flows into said second compartment;
• iii) optionally, mixing said substance with said sample;
• iv) moving said shutter of said second compartment whereby said substance flows into said third compartment and onto said sensor strip;
• and
• v) placing said cartridge in a reader adapted to detect a feature of said substance indicative of an item of interest in said sample and where said feature is recognized concluding said item of interest is present in said sample.