US 2026/0224734 A1 · METHOD FOR PREPARING MULTIFUNCTIONAL IMMUNO-AGONIST FOR REPROGRAMMING TUMOR-ASSOCIATED MACROPHAGES

Abstract

A multifunctional immuno-agonist for reprogramming tumor-associated macrophages and a method for its preparation are provided. A solid-phase carrier is constructed by coupling a siglec-10 receptor epitope to the surface of thiolated Fe3O4 magnetic beads through cysteine modification using a disulfide bond as the linkage. An amino acid-derived monomer is synthesized and polymerized on the carrier surface to form a functional polymer layer. A thiol-based reducing agent is then applied to remove the epitope template, yielding a polymer layer containing a specific recognition cavity. The resulting immuno-agonist is capable of binding to the siglec-10 receptor and anchoring macrophages through the recognition cavity, and the magnetic component enables localized accumulation under an external magnetic field. The material can promote macrophage polarization and modulate their immune activity. The immuno-agonist is designed for applications involving tumor-associated macrophages.

Claims8

Dependent claims

A method for preparing a multifunctional immuno-agonist for reprogramming tumor-associated macrophages, comprising:

preparing a mixed reaction solution of N-isopropylacrylamide, a functional monomer, N,N′-methylenebisacrylamide, and N-tert-butylacrylamide at a concentration of 13-65 mM, wherein a molar ratio of the N-isopropylacrylamide, the functional monomer, the N,N′-methylenebisacrylamide, and the N-tert-butylacrylamide is (32-98):(1-45):(1-5):(1-20);
adding a solid-phase carrier;
under continuous nitrogen flow, adding 1-10 mM ammonium persulfate, and performing an open-air stirring polymerization reaction at 38-45° C. for 8-16 hours, followed by magnetic separation to remove a first supernatant to obtain remaining nanoparticles;
and finally adding a 10-20 mM thiol reducing agent solution to the remaining nanoparticles and performing elution at 0-8° C. for 12-24 hours to obtain the multifunctional immuno-agonist as a product.

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Dependencies are inferred from the claim text.

Method for preparing multifunctional immuno-agonist for reprogramming tumor-associated macrophages

Life of this patent

Method for preparing multifunctional immuno-agonist for reprogramming tumor-associated macrophages

Life of this patent

Abstract

Claims8