Antibodies with selectivity for the 4ig isoform of b7-h3 and methods of use thereof
AssigneeBoard of Regents, The University of Texas System
InventorsSeth T. GAMMON, David PIWNICA-WORMS
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Abstract
Provided herein are antibodies and antigen binding portions thereof that specifically bind the 4Ig isoform of B7 homolog 3 protein (B7-H3), various compositions of such antibodies or antigen binding portions thereof, and recombinant nucleic acids encoding the antibodies and antigen binding portions thereof. Also provided are methods of using the antibodies or antigen binding portions thereof in therapeutics and diagnostics for cancer or inflammation.
Claims33
An isolated antibody or antibody fragment, comprising:
- a heavy chain variable region (VH) having at least 90% identity to SEQ ID NO:
- 3 and comprising a VHCDR1 amino acid sequence comprising SEQ ID NO:
- 9, 15, or 21, a VHCDR2 amino acid sequence comprising SEQ ID NO:
- 10, 16, or 22, and a VHCDR3 amino acid sequence comprising SEQ ID NO:
- 11, 17, or 23 and having a tryptophan at position 47, a methionine at position 48, a valine at position 68, and an arginine at position 72 of SEQ ID NO:
- 3;
- and
- a light chain variable region (VL) having at least 90% identity to SEQ ID NO:
- 4 and comprising a VLCDR1 amino acid sequence comprising SEQ ID NO:
- 12, 18, or 24, a VLCDR2 amino acid sequence comprising SEQ ID NO:
- 13, 19 or 25, and a VLCDR3 amino acid sequence comprising SEQ ID NO:
- 14, 20, or 26 and having an arginine at position 45 and a tyrosine at position 70 of SEQ ID NO:
- 4.
-3. (canceled)
-7. (canceled)
-14. (canceled)
(canceled)
(canceled)
A chimeric antigen receptor (CAR) protein comprising an antigen binding domain comprising a heavy chain variable region (VH) having at least 90% identity to SEQ ID NO:
- 1, SEQ ID NO:
- 2, or SEQ ID NO:
- 3 and comprising a VHCDR1 amino acid sequence comprising SEQ ID NO:
- 9, 15, or 21, a VHCDR2 amino acid sequence comprising SEQ ID NO:
- 10, 16, or 22, and a VHCDR3 amino acid sequence comprising SEQ ID NO:
- 11, 17, or 23;
- and
- a light chain variable region (VL) having at least 90% identity to SEQ ID NO:
- 4 and comprising a VLCDR1 amino acid sequence comprising SEQ ID NO:
- 12, 18, or 24, a VLCDR2 amino acid sequence comprising SEQ ID NO:
- 13, 19 or 25, and a VLCDR3 amino acid sequence comprising SEQ ID NO:
- 14, 20, or 26 and comprising an arginine corresponding to position 45 and a tyrosine corresponding to position 70 of SEQ ID NO:
- 4.
21-23. (canceled)
-30. (canceled)
32-33. (canceled)
35-38. (canceled)
41-49. (canceled)
54-57. (canceled)
59-68. (canceled)
-72. (canceled)
Dependencies are inferred from the claim text.