Chimeric antigen receptors (car) with intrinsically disordered regions and methods of use thereof

Abstract

Compositions and methods for improved Adoptive Cell Therapy (ACT) are provided. Compositions of CAR-T cells including CAR modified by fusion with one or more copies of a polypeptide including from between 50 and 500 amino acids derived from intrinsically disordered regions (IDRs) of proteins are described. CAR-IDR-T cells have improved condensation of CAR and T cell activation, and overall enhanced anti-tumor efficacy as compared with CAR-T cells lacking the IDR domain(s). In preferred embodiments, CAR-IDR fusion peptides include an IDR domain of any of SEQ ID NOs.1-6. The compositions and methods provide enhanced CAR-T cell therapy for cancer as well as other disease and disorders. Also disclosed are genetically modified cells and pharmaceutical compositions and methods of use thereof for treating subjects having diseases or disorders.

Dependencies are inferred from the claim text.