US 2026/0219488 A1 · OPTICAL IMAGING APPARATUS

Abstract

A method of using an optical imaging apparatus includes emitting light from a laser, tilting a leading phase front of the light relative to a propagation direction thereof, creating a gradient of the light at a specimen to provide a spatial-temporal relationship, rotating at least one of: the specimen or the gradient light part from an initial imaging orientation to a rotated imaging orientation, and reconstructing at least a two dimensional image of the specimen from initial and the rotated orientation images. In another aspect, an optical imaging includes a laser emitting light therefrom, a grating configured to tilt a leading phase front of the light relative to a propagation direction thereof and configured to locally create a gradient of the light at a specimen, a first spatial light modulator configured to shape a portion of the light for an initial imaging orientation, a second spatial light modulator configured to shape a portion of the light for a rotated imaging orientation, an actuator configured to rotate at least one of: the specimen or a gradient light directing optic, from the initial imaging orientation to the rotated imaging orientation, and a spectrometer and programmable controller configured to reconstruct at least a two dimensional image of the specimen from initial and the rotated orientation images.

Claims21

Dependent claims

A method of using an optical imaging apparatus, the method comprising:

(a) emitting light from a laser, using scattering of the light without fluorescence and without light absorption on a biological specimen;
(b) tilting a leading phase front of the light relative to a propagation direction thereof;
(c) creating a gradient of the light at the specimen to provide a spatial-temporal relationship;
(d) rotating at least one of:
the specimen or the gradient light part, from an initial imaging orientation to a rotated imaging orientation;
and
(e) reconstructing at least a two dimensional image of the specimen from initial and the rotated orientation images.

4 more elements

A method of using an optical imaging apparatus, the method comprising:

(a) emitting laser pulses;
(b) focusing the pulses onto a grating;
(c) dispersing the pulses from the grating;
(d) programming a phase mask onto a programmable spatial light modulator;
(e) tilting a leading phase front of the pulses relative to a propagation direction thereof, the phase mask on the programmable spatial light modulator causing the tilting, and the phase mask creating a pulse pair in a time domain, with a delay dependent on a frequency of a phase pattern;
(f) focusing different frequencies of the dispersed pulses onto a specimen to generate a gradient locally at the specimen using a refractive index;
(g) scattering the pulses off of the specimen;
(h) measuring interference signals between the gradient and a reference portion of at least one of the pulses with a spectrometer, to retrieve a one-dimensional projection of the specimen along an initial orientation;
(i) rotating at least one of:
(i) the specimen or (ii) the gradient emission direction toward the specimen, from the initial orientation to multiple different rotated orientations;
and
(j) automatically reconstructing a two or three dimensional image of the specimen from the initial and the rotated orientation measurements.

9 more elements

Dependencies are inferred from the claim text.

Optical imaging apparatus

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Optical imaging apparatus

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Abstract

Claims21