Reagent, kit and test method for testing glycosylated hemoglobin
AssigneeAcon Biotech (Hangzhou) Co., Ltd. Leadway (Hk) Limited
InventorsYan QIN, Kexiang GAO, Yulong SUN, Li ZHANG
Life of this patent
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Abstract
The present invention provides a kit and a test method for testing glycated hemoglobin. The kit includes a test card, a reagent R1, and a reagent R2, where the test card includes a filtration membrane, the reagent R1 includes a buffer system, a surfactant, and an active reactant, and the reagent R2 includes a buffer system and a surfactant. In the present invention, by combining different types of surfactants in a certain proportion, a reagent for determining glycated hemoglobin has good thermal stability and can be transported and preserved at normal temperature.
Claims22
A kit for determining glycated hemoglobin, comprising:
- a filtration membrane for retaining precipitated hemoglobin;
- a reagent R1 comprising a boronic acid conjugate, a zinc ion, and a surfactant;
- and
- a reagent R2 comprising a buffer solution and a surfactant,
- wherein the surfactant in the reagent R1 comprises a nonionic surfactant and an anionic surfactant.
A method for determining glycated hemoglobin, comprising:
- mixing a blood sample with a reagent R1 to form a mixed sample;
- passing the mixed sample through a filtration membrane capable of retaining precipitated hemoglobin;
- rinsing a filtration membrane retaining the precipitated hemoglobin with a reagent R2;
- and
- separately detecting amounts of the glycated hemoglobin and total hemoglobin, and in turn calculating a percentage of the glycated hemoglobin,
- wherein
- the reagent R1 comprises a boronic acid conjugate, a zinc ion, and a surfactant;
- and
- the reagent R2 comprises a buffer solution and a surfactant,
- wherein the surfactant in the reagent R1 comprises a nonionic surfactant and an anionic surfactant.
A reagent for determining glycated hemoglobin, comprising a reagent R1 and a reagent R2, wherein the reagent R1 comprises an active reactant, a metal ion, and a surfactant, and the reagent R2 is an eluent, wherein the surfactant in the reagent R1 comprises a nonionic surfactant and an anionic surfactant.
A method for preserving a reagent, wherein the reagent comprises a boronic acid conjugate, a zinc ion, and a surfactant, wherein the surfactant comprises a nonionic surfactant and an anionic surfactant.
Dependencies are inferred from the claim text.