In vitro diagnostic method for detecting the presence of a target by using stabilized membrane proteins
AssigneeCUBE BIOTECH GmbH
InventorsBarbara Maertens, Jan Kubicek, Roland Fabis, Philipp Timo Hanisch, Sergej Balanda
Life of this patent
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Abstract
The technology pertains to in vitro diagnostic methods, kits, membrane proteins and test devices for detecting the presence and/or absence of a target in a biological sample, wherein said target binds to at least one epitope of an isolated membrane protein, or at least to a fragment of said isolated membrane protein comprising at least one epitope of said isolated membrane protein binding to said target.
Claims29
An in vitro diagnostic method for detecting the presence and/or absence of a target in a biological sample, wherein said target binds to at least one epitope of an isolated membrane protein, or at least to a fragment of said isolated membrane protein comprising at least one epitope of said isolated membrane protein binding to said target, wherein the method comprises:
- (a) contacting said biological sample with said isolated membrane protein, or said fragment thereof, wherein the isolated membrane protein, or said fragment thereof is solubilized and stabilized by a polymer and/or copolymer, in particular in a polymer and/or copolymer nanodisc;
- and
- (b) detecting the formation of a target-protein complex comprising said target and said membrane protein, or said fragment thereof, in the biological sample.
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The method according to claim wherein the target is a target virus or a fragment thereof in a biological sample, and wherein said virus or said virus fragment comprises a viral attachment protein that binds to at least one epitope of said membrane protein, or at least to a fragment of said membrane protein.
An in vitro diagnostic kit for detecting the presence and/or absence of a target in a biological sample:
- (a) at least an isolated membrane protein, or a fragment thereof comprising at least one epitope binding to a target, wherein said isolated membrane protein or said fragment thereof is in the presence of a polymer and/or copolymer that can solubilize and stabilize said membrane protein, in particular the membrane protein is comprised in a polymer and/or copolymer nanodisc;
- and
- (b) reagents for detecting the formation of a target-protein complex between said target and said membrane protein, or said fragment thereof, in said biological sample, wherein said isolated membrane protein or fragment thereof and said reagents are present in an amount sufficient to detect the formation of said target-protein complex.
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24-44. (canceled)
A test device for the early and rapid detection of a target in a biological sample, wherein the device comprises a test strip, wherein the test strip comprises;
- at least one sample application site, in particular a sample pad;
- at least one test zone and one control zone, and
- at least a zone as a conjugate pad comprising gold protein-conjugates,
- wherein in the test zone and/or the conjugate pad comprises immobilized isolated membrane proteins, or fragments thereof, wherein the isolated membrane proteins, or said fragments thereof are solubilized and stabilized by a polymer and/or copolymer, in particular in a polymer and/or copolymer nanodisc.
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49-66. (canceled)
Dependencies are inferred from the claim text.