Bioassay method for hangeshashinto using pharmacological activity as indicator
AssigneeTsumura & Co.
InventorRyota IMAI
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Abstract
A bioassay method for evaluating pharmacological activity which can be used for quality control of hangeshashinto is provided. A bioassay method for pharmacological activity of hangeshashinto characterized by adding an inflammatory cytokine a and hangeshashinto-containing test sample to a human oral keratinocyte, culturing the human oral keratinocyte, and then measuring the prostaglandin E2 concentration of the culture supernatant, and a quality control method of hangeshashinto using the bioassay method.
Claims13
A bioassay method for pharmacological activity of hangeshashinto, comprising:
- adding an inflammatory cytokine and a hangeshashinto-containing test sample to a human oral keratinocyte;
- culturing the human oral keratinocyte;
- and
- then measuring the prostaglandin E2 concentration of the culture supernatant.
A quality control method of hangeshashinto, comprising the following (a), (b), and (d):
- (a) a step of adding an inflammatory cytokine to a human oral keratinocyte, culturing the human oral keratinocyte, and then measuring the prostaglandin E2 concentration of the culture supernatant;
- (b) a step of adding the inflammatory cytokine and a hangeshashinto-containing test sample to a human oral keratinocyte, culturing the human oral keratinocyte, and then measuring the prostaglandin E2 concentration of the culture supernatant;
- and
- (d) comparing the prostaglandin E2 concentrations measured in (a) and (b).
A quality control method of hangeshashinto, comprising the following (a) to (c) and (e):
- (a) adding an inflammatory cytokine to a human oral keratinocyte, culturing the human oral keratinocyte, and then measuring the prostaglandin E2 concentration of the culture supernatant;
- (b) adding the inflammatory cytokine and a hangeshashinto-containing test sample to a human oral keratinocyte, culturing the human oral keratinocyte, and then measuring the prostaglandin E2 concentration of the culture supernatant;
- (c) adding the inflammatory cytokine and a nonsteroidal anti-inflammatory agent as a positive control to a human oral keratinocyte, culturing the human oral keratinocyte, and then measuring the prostaglandin E2 concentration of the culture supernatant;
- and
- (e) comparing the prostaglandin E2 concentrations measured in (a) to (c).
Dependencies are inferred from the claim text.