Method for quantifying insulin secretion capacity in human cell
AssigneeJuntendo Educational Foundation
InventorsHideki ENDOH, Masahito MATSUMOTO, Yasushi OKAZAKI
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Abstract
A method to rapidly and inexpensively quantify human insulin secreted from human cells. This method for quantifying insulin secretion capacity in a human cell involves culturing a recombinant cell and measuring an amount of a secreted fused C-peptide. The recombinant cell is prepared by replacing an insulin gene in the genome of a human cultured cell line with a gene which expresses a fused C-peptide having a detectable tag peptide inserted in a C-peptide chain in a human proinsulin molecule.
Claims17
A method for quantifying insulin secretion capacity in a human cell, the method comprising:
- culturing a recombinant cell;
- and
- measuring an amount of a secreted fused C-peptide,
- wherein the recombinant cell is prepared by replacing an insulin gene in the genome of a human cultured cell line with a gene which expresses a fused C-peptide having a detectable tag peptide inserted in a C-peptide chain in a human proinsulin molecule.
A recombinant cell prepared by replacing an insulin gene in the genome of a human cultured cell line with a gene which expresses a fused C-peptide having a detectable tag peptide inserted in a C-peptide chain in a human proinsulin molecule.
An insulin production and secretion promoter, comprising one or two members selected from the group consisting of valproic acid and zebularine.
Dependencies are inferred from the claim text.