Fractionation method and fractionation apparatus
AssigneeShimadzu Corporation
InventorKazuhiro KAWAKAMI
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Abstract
A method for fractionating a target component from a measurement sample comprises a separation step of separating the target component, a trapping step of trapping the separated target component in a trap column, an elution step of causing the target component to flow out from the trap column, and a recovery step of recovering the target component that has flowed out from the trap column, wherein, in the elution step, water and the target component discharged from the trap column are separated by adjusting an inflow rate of an elution organic solvent into the trap column.
Claims5
A fractionation method for fractionating a target component from a measurement sample, comprising:
- a separation step of separating the target component by passing the measurement sample together with a mobile phase through a separation column;
- a trapping step of trapping the separated target component in a trap column;
- an elution step of causing the target component to flow out from the trap column by causing an elution organic solvent to flow into the trap column;
- and a recovery step of recovering the target component that has flowed out from the trap column, wherein, in the elution step, water and the target component discharged from the trap column are separated by adjusting an inflow rate of the elution organic solvent into the trap column.
Dependencies are inferred from the claim text.