Device and method for detecting molecules

Device for detecting molecules comprising:

• a substrate comprising a first face and a second face, opposite each other, the substrate comprising at least one through channel having a first opening at the first face and a second opening at the second face, the channel being delimited by a side wall extending between the first and the second face,
• a receptacle configured to contain a saline solution and the molecule to be detected, the substrate being insertable in the receptacle to be immersed in the saline solution being used,
• a light source configured to illuminate the channel with a monochromatic electromagnetic radiation such that a hotspot is generated within the channel,
• a generator configured to generate a potential difference between the first face and the second face of the substrate such that during use the molecule to be detected in the channel is moved between the first and the second opening,
• wherein
• the device comprises a particle at least in part inserted in the channel,
• the generable hotspot is located between the particle and the side wall of the channel,
• the particle and the side wall are spaced so that the molecule to be detected, which emits an optically or electrically detectable detection signal when crossing the hotspot, flows between the particle and the side wall at the hotspot.