Systems for upconversion chemiluminescence detection based on magnetic microparticles

A system for upconversion chemiluminescence detection based on magnetic microparticles, comprising:

• a working surface;
• wherein the working surface includes:
• a sample carrying module configured to input, preload, and output a plurality of samples;
• a gripping module configured to grip a reaction cup and a pipette tip from a previous working position to a next working position;
• a sample aspiration and dispensing module configured to aspirate at least one of the plurality of samples and reagents and dispense the at least one of the plurality of samples and the reagents into the reaction cup, wherein the reagents include at least a monoclonal antibody reagent bound with magnetic microparticles and a monoclonal antibody reagent labeled with up-converting phosphor;
• a detection preparation module configured to perform one or more cycles of mixing, incubation, and a plurality of cycles of washing and separation on the at least one of the plurality of samples and the reagents dispensed into the reaction cup;
• and
• an optical measurement module configured to excite an up-converting luminescent label and detect a fluorescence signal intensity.