Compositions, kits and methods for detection of viral sequences

A composition for multiplex detection of viral monkeypox nucleic acid and viral orthopox nucleic acid, the composition comprising:

• a first primer-probe set including
• a first forward primer and a first reverse primer each configured to hybridize to different ends of a first target nucleic acid sequence present in a target region of monkeypox viral DNA, to a DNA copy of the first target nucleic acid sequence, or to a complement of the first target nucleic acid sequence or a DNA copy of the complement, and
• a first probe configured to hybridize to a first target nucleic acid subsequence that is complementary or identical to the first target nucleic acid sequence, or to a DNA copy of the first target nucleic acid subsequence,
• wherein the first primer-probe set includes at least one nucleic acid sequence selected from SEQ ID NOs:
• 16-180;
• a second primer-probe set including
• a second forward primer and a second reverse primer each configured to hybridize to different ends of a second target sequence present in a target region of orthopox viral DNA, to a DNA copy of the second target nucleic acid sequence, or to a complement of the second target nucleic acid sequence or a DNA copy of the complement, and
• a second probe configured to hybridize to a second target nucleic acid subsequence that is complementary or identical to the second target nucleic acid sequence, or to a DNA copy of the second target nucleic acid subsequence,
• wherein the second primer-probe set includes at least one nucleic acid sequence selected from SEQ ID NOs:
• 4-12.