Markers to predict macrocyclic lactone drug resistance in dirofilaria immitis, the causative agent of heartworm disease
AssigneesElanco US Inc., McGill University
InventorsRoger K. Prichard, Catherine Bourguinat, Timothy G. Geary
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Abstract
Disclosed are nucleic acid molecules from the genome of Dirofilaria spp. nematodes that contain single nucleotide polymorphisms related to reduced responsiveness of the nematodes to macrocyclic lactones. In one example, the species of Dirofilaria is Dirofilaria immitis (the agent of heartworm in animals). Also disclosed are methods for determining the responsiveness of Dirofilaria spp. nematodes to macrocyclic lactones, methods for selecting a treatment to treat an animal infected with Dirofilaria spp. nematode, and kits for determining the responsiveness of Dirofilaria spp. nematodes to macrocyclic lactones.
Claims20
A method for determining the responsiveness of a Dirofilaria spp. nematode to a macrocyclic lactone, the method comprising determining the genotype of the nematode at a polymorphic site in a nucleic acid molecule that includes one or more of SEQ ID NOs:
- 1-127 from the nematode.
An isolated nucleic acid molecule comprising one or more of SEQ ID NOs:
- 1-127.
A kit for determining the responsiveness of a Dirofilaria spp. nematode to a macrocyclic lactone, the kit comprising:
- a probe capable of determining the genotype of the nematode at a polymorphic site of one or more of SEQ ID NOs:
- 1-127.
Dependencies are inferred from the claim text.