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AssigneePacific Biosciences of California, Inc.
InventorsMorassa Mohseni MIDDLETON, Mark C. WALLEN, Pinar IYIDOGAN, Michael James SCHMIDT, Brittany A. ROHRMAN, Ying Lin LIU, Fabian BLOCK, Arnold OLIPHANT
Estimating the term…
Methods, compositions, kits and apparatuses that include a fluid, the fluid containing a ternary complex and Li+, wherein the ternary complex includes a primed template nucleic acid, a polymerase, and a nucleotide cognate for the next correct base for the primed template nucleic acid molecule. As an alternative or addition to Li+, the fluid can contain betaine or a metal ion that inhibits polymerase catalysis such as Ca2+. In addition to Li+, the fluid can contain polyethylenimine (PEI) with or without betaine.
A composition, comprising a fluid, the fluid comprising a ternary complex and Li+, wherein the ternary complex comprises a primed template nucleic acid molecule, a polymerase, and a nucleotide cognate for the next correct base for the primed template nucleic acid molecule.
A method of detecting a primed template nucleic acid, comprising the steps of:
Dependencies are inferred from the claim text.