US 2026/0218271 A1 · MODIFIED IMMUNO-MOLECULAR ASSAY FOR FASTER BIOMARKER DETECTION

Abstract

The present invention relates to an improved immuno-molecular assay, for example a Proximity Extension Assay protocol or a Proximity Ligation Assay (PLA) protocol, which is more compatible with the assay durations that are expected in the context of point-of-care diagnostics. In particular, the time needed for performing the Proximity Extension Assay protocol of the invention is reduced by more than 13-folds as compared to classical Proximity Extension Assay protocols, without significantly affecting the performance of the assay for protein detection. Thus, the method of the invention provides a more rapid and simpler procedure to screen and diagnose diseases in a non-invasive way. The use of such a method in an integrated system is an exciting and expected improvement in clinical diagnostics for the detection of biomarkers in human samples.

Claims15

Dependent claims

An immuno-molecular assay for detecting a protein analyte or a fragment thereof in a biological sample, the assay comprising the steps of:

A) contacting the biological sample with at least one molecule comprising a nucleic acid domain and a binding domain being able to directly or indirectly bind to the protein analyte,
B) incubating the resulting sample from 1 to 30 minutes under intermittent shaking for allowing the at least one molecule to bind to the protein analyte,
C) detecting the binding by a molecular detection method.

Dependencies are inferred from the claim text.

Modified immuno-molecular assay for faster biomarker detection

Life of this patent

Modified immuno-molecular assay for faster biomarker detection

Life of this patent

Abstract

Claims15