Enzyme, strain for producing salidroside, and production method

A strain for producing salidroside, wherein the strain comprises a gene encoding glucosyltransferase U8GT3 and a gene encoding tyrosine decarboxylase,

• preferably, the gene encoding tyrosine decarboxylase is a gene encoding a poppy-derived tyrosine decarboxylase or a gene encoding a poppy-derived tyrosine decarboxylase mutant;
• more preferably,
• the glucosyltransferase U8GT3 comprises an amino acid sequence represented by SEQ ID NO.10;
• the poppy-derived tyrosine decarboxylase comprises an amino acid sequence represented by SEQ ID NO.8;
• and
• the poppy-derived tyrosine decarboxylase mutant is a tyrosine decarboxylase in which the tyrosine at position 350 is mutated to phenylalanine, and comprises an amino acid sequence represented by SEQ ID NO.7,
• most preferably, the yeast is Saccharomyces cerevisiae.

A biomaterial, wherein the biomaterial is any one of the following:

• A1) a nucleic acid molecule encoding a poppy-derived tyrosine decarboxylase and/or a nucleic acid molecule encoding a tyrosine decarboxylase mutant;
• A2) an expression cassette comprising the nucleic acid molecule described in A1);
• A3) a recombinant vector comprising the nucleic acid molecule described in A1), or a recombinant vector comprising the expression cassette described in A2);
• preferably,
• the tyrosine decarboxylase mutant comprises an amino acid sequence represented by SEQ ID NO.7;
• and
• the poppy-derived tyrosine decarboxylase comprises an amino acid sequence represented by SEQ ID NO.8;
• more preferably,
• the tyrosine decarboxylase mutant comprises a tyrosine decarboxylase mutant expressed by a nucleic acid molecule represented by SEQ ID NO.1;
• and
• the poppy-derived tyrosine decarboxylase comprises a tyrosine decarboxylase expressed by a nucleic acid molecule represented by SEQ ID NO.2;
• or
• wherein the biomaterial is any one of the following:
• B1) a nucleic acid molecule encoding glucosyltransferase U8GT3;
• B2) an expression cassette comprising the nucleic acid molecule described in B1);
• B3) a recombinant vector comprising the nucleic acid molecule described in B1), or a recombinant vector comprising the expression cassette described in B2);
• preferably,
• the glucosyltransferase U8GT3 comprises an amino acid sequence represented by SEQ ID NO.10;
• more preferably,
• the glucosyltransferase U8GT3 comprises glucosyltransferase U8GT3 expressed by a nucleic acid molecule represented by SEQ ID NO.4.