Rna polymerases
AssigneeNew England Biolabs, Inc.
InventorsJackson A. Buss, Vladimir Potapov, Peter R. Weigele, Jennifer Ong, Lili Mitchell, Dillon B. Nye, Tien-Hao Chen, G. B. Robb
Life of this patent
Estimating the term…
Abstract
The present disclosure relates, according to some embodiments, to RNA polymerases and fusions, compositions, kits, methods, and workflows including RNA polymerases. According to some embodiments, an RNA polymerase may include an H6 RNA polymerase (e.g., SEQ ID NO: 21) and/or variants thereof (e.g., variants having ≥90%, ≥95%, ≥98% to any of SEQ ID NOS: 1-20 and/or 22).
Claims27
A variant H6 RNA polymerase having (a) RNA polymerase activity, and (b) an amino acid sequence having (i) at least 98% identity to any of SEQ ID NOS:
- 1-20, and (ii) at least one substitution relative to SEQ ID NO:
- 21.
A variant H6 RNA polymerase having (a) RNA polymerase activity, and (b) an amino acid sequence having (i) at least 95% identity to any of SEQ ID NOS:
- 1-20, and (ii) at least one substitution relative to SEQ ID NO:
- 21 at a position selected from positions corresponding to positions 106, 108, 134, 215, 232, 283, 327, 329, 378, 402, 419, 454, 459, 480, 481, 484, 490, 516, 519, 538, 715, 717, 756, 759, 798, 803, and 848 of SEQ ID NO:
- 21.
A variant H6 RNA polymerase having (a) RNA polymerase activity, and (b) an amino acid sequence having (i) at least 95% identity to any of SEQ ID NOS:
- 1-20, and (ii) at least one substitution relative to SEQ ID NO:
- 21 at a position selected from positions corresponding to positions 215, 459, 538, 759, 798, 803, and 848 of SEQ ID NO:
- 21.
A variant H6 RNA polymerase having (a) RNA polymerase activity, and (b) an amino acid sequence having (i) at least 95% identity to any of SEQ ID NOS:
- 1-20, and (ii) substitutions relative to SEQ ID NO:
- 21 at positions corresponding to positions 215, 459, 538, 759, 798, 803, and 848 of SEQ ID NO:
- 21.
A cell-free H6 RNA polymerase having an amino acid sequence at least 95% identical to SEQ ID NO:
- 21.
Dependencies are inferred from the claim text.