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US 2026/0218261 A1Published application
Enzymatic dna synthesis
AssigneeThe J. David Gladstone Institutes, A Testamentary Trust Established Under the Will of J. David
InventorsSeth Shipman, Christina Palka
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Abstract
Provided herein are methods for enzymatically producing de novo DNA molecules.
Claims19
A method to synthesize a single strand DNA segment comprising
- a) contacting an AbiK and/or Abi-P2 enzyme with a nucleotide, wherein the enzyme and the nucleotide form a complex;
- b) removing any unbound nucleotide from a);
- c) building the single strand DNA segment from the 3″ end of the nucleotide bound to the enzyme/nucleotide of b) by contacting the enzyme/nucleotide of b) with another nucleotide;
- d) removing any unbound nucleotide from c);
- and
- e) repeating the steps of contacting and removing free nucleotides until a desired length and sequence of the single strand DNA segment is formed.
3 more elements
Dependencies are inferred from the claim text.