Galactose-1-phosphate uridyltransferase mutant and use thereof in preparation of l-lysine
AssigneeNingxia Eppen Biotech Co., Ltd
InventorsXiaoqun ZHOU, Gang MENG, Aiying WEI, Chunguang ZHAO, Fengyong MA, Ying ZHANG
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Abstract
Provided are a galactose-1-phosphate uridyltransferase mutant and the use thereof in the preparation of L-lysine. The galactose-1-phosphate uridyltransferase is A1) or A2): A1) a protein having an amino acid sequence as shown in SEQ ID No. 2; and A2) a fusion protein obtained by linking a tag to the N-terminal or/and the C-terminal of A1). Terminating or knocking out coding genes of the galactose-1-phosphate uridyltransferase in advance can improve the yield of L-lysine in cells.
Claims12
A method for preparation of L-lysine, comprising applying a substance for knocking out a protein coding gene or inhibiting the protein content or activity;
- the protein comprisesA1) or A2) as follows:
- A1) a protein having an amino acid sequence of SEQ ID No. 2;
- and
- A2) a fusion protein obtained by linking a tag to an N-terminal or/and a C-terminal of A1).
(canceled)
Dependencies are inferred from the claim text.