An engineered rice with reduced vacuolar proton pyrophosphatase 5 (vpp5) expression
AssigneeBoard of Trustees of the University of Arkansas
InventorsVibha SRIVASTAVA, Peter James ICALIA GANN
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Abstract
A genetically engineered Oryza sativa seed is disclosed. The seed is genetically engineered so that the RNA expression or protein expression of vacuolar proton pyrophosphatase 5 (VPP5) in the developing seed is reduced by at least 50% as compared to a non-engineered control seed. A method of downregulating VPP5 in a tissue culture of regenerable plant cells or protoplasts is also disclosed.
Claims25
A genetically engineered Oryza sativa subsp japonica seed, wherein the RNA expression or protein expression of vacuolar proton pyrophosphatase 5 (VPP5) in the developing seed is reduced by at least 50% as compared to a non-engineered control seed.
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A method of downregulating VPP5 in Oryza sativa comprising:
- providing an Oryza sativa seed;
- inducing the seed to form a callus;
- introducing a targeting nucleic acid into the callus, wherein the nucleic acid comprises a sequence complementary to a gene encoding VPP5 or a transcription regulatory region of the gene encoding VPP5;
- introducing a nuclease into the callus, wherein the nuclease causes a change in the nucleic acid sequence of the gene encoding VPP5 or the transcription regulatory region of the gene encoding VPP5 that is targeted by the nucleic acid;
- propagating the callus, wherein propagation of the callus produces genetically engineered Oryza sativa plants;
- and
- selecting the genetically engineered Oryza sativa plants for reduced VPP5 expression, and optionally wherein the rice is subsp. japonica.
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Dependencies are inferred from the claim text.