Regulatory nucleic acid molecules for enhancing gene expression in plants
AssigneeBASF SE
InventorsFrank MEULEWAETER, Christophe LISERON-MONFILS
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Abstract
The present invention is in the field of plant molecular biology and provides methods for production of high expressing promoters and the production of plants with enhanced expression of nucleic acids wherein nucleic acid expression enhancing nucleic acids (NEENAs) are functionally linked to said promoters and/or introduced into plants.
Claims22
1-17. (canceled)
A recombinant expression construct comprising one or more nucleic acid expression enhancing nucleic acid (NEENA) molecules, wherein said one or more NEENA is selected from:
- i) the nucleic acid molecule having the sequence of SEQ ID NO:
- 17,
- ii) a nucleic acid molecule having the sequence of SEQ ID NO:
- 17, wherein:
- the nucleotide corresponding to position 63 of SEQ ID NO:
- 17 is an A, a G, or a C nucleotide;
- the nucleotide corresponding to position 64 of SEQ ID NO:
- 17 is an A, a G, or a C nucleotide;
- the nucleotide corresponding to position 67 of SEQ ID NO:
- 17 is an A, a G, or a C nucleotide;
- the nucleotide corresponding to position 68 of SEQ ID NO:
- 17 is a G, an A, or a T nucleotide;
- the nucleotide corresponding to position 70 of SEQ ID NO:
- 17 is a T or an A nucleotide;
- the nucleotide corresponding to position 71 of SEQ ID NO:
- 17 is a C, an A, or a T nucleotide;
- which has expression enhancing activity as the corresponding nucleic acid molecule having the sequence of SEQ ID NO:
- 17, or
- iii) the nucleic acid molecule which is the complement of any of the nucleic acid molecules of i) or ii),
- functionally linked to one or more promoters and wherein the promoter is heterologous to said one or more NEENA molecule.
A transgenic cell or transgenic plant or part thereof comprising one or more heterologous NEENA molecules, selected from:
- i) the nucleic acid molecule having the sequence of SEQ ID NO:
- 17,
- ii) a nucleic acid molecule having the sequence of SEQ ID NO:
- 17, wherein:
- the nucleotide corresponding to position 63 of SEQ ID NO:
- 17 is an A, a G, or a C nucleotide;
- the nucleotide corresponding to position 64 of SEQ ID NO:
- 17 is an A, a G, or a C nucleotide;
- the nucleotide corresponding to position 67 of SEQ ID NO:
- 17 is an A, a G, or a C nucleotide;
- the nucleotide corresponding to position 68 of SEQ ID NO:
- 17 is a G, an A, or a T nucleotide;
- the nucleotide corresponding to position 70 of SEQ ID NO:
- 17 is a T or an A nucleotide;
- the nucleotide corresponding to position 71 of SEQ ID NO:
- 17 is a C, an A, or a T nucleotide;
- which has expression enhancing activity as the corresponding nucleic acid molecule having the sequence of SEQ ID NO:
- 17, or
- iii) the nucleic acid molecule which is the complement of any of the nucleic acid molecules of i) or ii).
A method for enhancing expression derived from a plant promoter comprising functionally linking to a promoter one or more nucleic acid expression enhancing nucleic acid (NEENA) molecule heterologous to said promoter, wherein said one or more NEENA is selected from:
- i) the nucleic acid molecule having the sequence of SEQ ID NO:
- 17,
- ii) a nucleic acid molecule having the sequence of SEQ ID NO:
- 17, wherein:
- the nucleotide corresponding to position 63 of SEQ ID NO:
- 17 is an A, a G, or a C nucleotide;
- the nucleotide corresponding to position 64 of SEQ ID NO:
- 17 is an A, a G, or a C nucleotide;
- the nucleotide corresponding to position 67 of SEQ ID NO:
- 17 is an A, a G, or a C nucleotide;
- the nucleotide corresponding to position 68 of SEQ ID NO:
- 17 is a G, an A, or a T nucleotide;
- the nucleotide corresponding to position 70 of SEQ ID NO:
- 17 is a T or an A nucleotide;
- the nucleotide corresponding to position 71 of SEQ ID NO:
- 17 is a C, an A, or a T nucleotide;
- which has expression enhancing activity as the corresponding nucleic acid molecule having the sequence of SEQ ID NO:
- 17, or
- iii) the nucleic acid molecule which is the complement of any of the nucleic acid molecules of i) or ii).
Dependencies are inferred from the claim text.