Production of canthaxanthin
AssigneeAmyris, Inc.
InventorsChristopher J. PADDON, Victor F. HOLMES, Matthew E. JONES, Yoseph TSEGAYE
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Abstract
Provided herein are compositions and methods for production and recovery of canthaxanthin from a recombinant cell.
Claims25
A genetically modified host cell capable of producing canthaxanthin comprising a nucleic acid capable of expressing Bradyrhizobium β-carotene ketolase.
The genetically modified host cell of any of the previous claims, which further comprises a nucleic acid capable of expressing Paracoccus β-carotene ketolase.
The genetically modified host cell of any of the previous claims, which further comprises
- (a) a nucleic acid capable of expressing a GGPP synthase;
- (b) a nucleic acid capable of expressing a phytoene synthase;
- (c) a nucleic acid capable of expressing a phytoene desaturase;
- and/or
- (d) a nucleic acid capable of expressing a lycopene cyclase.
The genetically modified host cell of any of the preceding claims, wherein each β-carotene ketolase, GGPP synthase, phytoene synthase, phytoene desaturase, and/or lycopene cyclase is expressed from an inducible promoter.
The genetically modified host cell of claim 14, wherein the inducible promoter is a GAL promoter.
The genetically modified host cell of any of the previous claims which further comprises a gas4 deletion.
The genetically modified host cell of any of the previous claims which further comprises a nucleic acid encoding a polyprenyl synthase for producing a polyprenyl diphosphate.
The genetically modified host cell of any of the previous claims which further comprises a nucleic acid encoding a FPP synthase.
The genetically modified host cell of any of the previous claims which further comprises one or more nucleic acids encoding one or more enzymes of the mevalonate pathway.
The genetically modified host cell of any of the previous claims which further comprises one or more nucleic acids encoding all of the enzymes of the mevalonate pathway.
The genetically modified host cell of any of the previous claims which is a microbial cell.
The genetically modified host cell of any of the previous claims which is a yeast.
The genetically modified host cell of any of the previous claims which is Saccharomyces cerevisiae.
A composition comprising the genetically modified host cell of any of the previous claims.
A method for producing canthaxanthin comprising the steps:
- (a) culturing a population of the host cells of any one of the previous claims in a medium with a carbon source under conditions suitable for making canthaxanthin to yield a culture broth;
- and
- (b) recovering said canthaxanthin from the culture broth.
Dependencies are inferred from the claim text.