Heterologous production of the c33–c45 polyketide fragment of anticancer apratoxins in a cyanobacterial host
AssigneesUniversity of Florida Research Foundation, Inc., Smithsonian Institution
InventorsYousong Ding, Hendrik Luesch, Valerie J. Paul
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Abstract
The present disclosure relates to recombinant cyanobacterial cells for the production of a chemical compound of interest. In particular, the present disclosure relates to genetic modifications that introduce one or more heterologous polyketide synthases (PKS) into a cyanobacterial cell. These cells can further comprise nucleic acid constructs that provide the cyanobacterial cells with the capability of producing chemicals of interest or compounds of interest, including Compound (I).
Claims30
A recombinant cyanobacterial cell comprising a nucleic acid construct comprising a nucleic acid sequence encoding a heterologous polyketide synthase (PKS);
- and a nucleic acid sequence encoding a type-II thioesterase (TE-II).
A recombinant cyanobacterial cell comprising an expression construct comprising an inducible promoter operably linked to a nucleic acid sequence encoding, in the following order:
- Moorena bouillonii AprA, AprB, AprC, AprD, AprE, AprF, AprG;
- and
- a type-II thioesterase (TE-II) from an erythromycin cluster.
An expression construct comprising a promotor operably linked to a nucleic acid sequence encoding, in the following order:
- Moorena bouillonii AprA, AprB, AprC, AprD, AprE, AprF, AprG;
- and
- a type-II thioesterase (TE-II) from an erythromycin cluster.
A recombinant cyanobacterial cell comprising at least one nucleic acid construct that provides for production of Compound (I):
- or a salt thereof.
Dependencies are inferred from the claim text.