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US 2026/0218207 A1Published application
Fusion polypeptides
AssigneeDanisco US Inc.
InventorsJimmy CHAN, David A. ESTELL, Amy Deming LIU, Michael C. MILLER, Igor NIKOLAEV
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Abstract
Provided herein, inter alia, are fusion DNA constructs comprising improved protease recognition sequences for expressing and purifying one or more polypeptides of interest as well as methods for producing one or more polypeptides of interest in a recombinant host cell.
Claims21
A fusion polypeptide comprising an amino acid sequence T1-S2-V-A3-V4-E5-X1-X2-Q6-V7- (SEQ ID NO:1), wherein 1) X1 and X2 are basic amino acids;
- and 2) the amino acid sequence has one or more substitution(s) selected from the group consisting of:
- a. T1 substituted with an amino acid selected from the group consisting of a hydrophobic amino acid, an aromatic amino acid, an acidic amino acid, and a basic amino acid;
- b. S2 substituted with an amino acid selected from the group consisting of a hydrophobic amino acid, an aromatic amino acid, a basic amino acid, and an amino acid that influences chain orientation;
- c. A3 substituted with an amino acid selected from the group consisting of a basic amino acid and M;
- d. V4 substituted with L;
- e. E5 substituted with an aromatic amino acid;
- f. Q6 substituted with an amino acid selected from the group consisting of an acidic amino acid and an amino acid that influences chain orientation;
- and/or
- g. V7 substituted with an amino acid selected from the group consisting of L, I, and an aromatic amino acid
6 more elements
The fusion polypeptide of claim 12, wherein T8 is substituted with an E or F.
21-73. (canceled)
Dependencies are inferred from the claim text.